seawater for 1 l
medium, pH 10] after incubation at 25 uC. When the colour
of the pH indicator, BTB, changed, a small portion of culture
was transferred to new medium. Strain Shu-P-Ggiii25-2T was
isolated from the second enrichment culture on GYPiii agar
after incubation at 25 uC for three days. Isolate Shu-PGgiii25-2T
was routinely cultivated on trypticase soy agar
(TSA; BBL) containing 50 % Herbst’s artificial seawater and
incubated at 27 uC. Herbst’s artificial seawater contains the
following