Waste of vinegar fermentation was supplied by Kukrer
A.S (Turkey). 1 ml samples were incubated statically at pH
6.0 and 30ºC for 7 days in Hestrin-Schramm (HS) medium
(30 ml) composed of 2.0% D-glucose, 0.5% peptone, 0.5%
yeast extract, 0.27% Na2HPO4 and 0.115 citric acid [16]. In
case of microbial growth, the cultures were streaked onto
HS-agar (1.5% agar) plates.