การผลิตสารลดแรงตึงผิวชีวภาพจากแบคทีเรีย were isolated from soil samples contaminated with petroleum hydrocarbons. Isolates were screened for biosufactant production using Cetyl Tri Ammonium Bromind (CTAB) – Methylend blue agar selection medium . A candidates bacterial strain was selected for further studies based on surface tension reduction capacity and rapid drop collapse activity. Biochemical characteristics and partially sequenend 16S rRNA gene of the isotate, 5B, identified the bacterium as Bacillus clausii.
Different carbon and nitrogen sources were evaluated for their effect on biosurfactant production.