6. Incubate for 10–20 minutes at room temperature. Mix the tube occasionally by gentle inversion. 7. Centrifuge at 2000 × g for 5 minutes at 4°C. Remove and discard supernatant.
6. Incubate for 10–20 minutes at room temperature. Mix thetube occasionally by gentle inversion.7. Centrifuge at 2000 × g for 5 minutes at 4°C. Remove anddiscard supernatant.