MATERIALS AND METHODS
Subjects
Subjects included in this study were 10 diabetic
patients attending the Out-Patient Department
of Naresuan University Hospital as part of their follow-
up routine and 10 healthy blood donors (aged
20-36 years) from the Blood Bank Centre, Naresuan
University. The diagnosis of diabetes was established
by recording fasting blood sugar levels ≥ 125
mg/dl two times. Nine patients were diagnosed with
non-insulin dependent (type 2) diabetes mellitus and
one patient with insulin-dependent (type 1) diabetes
mellitus. Eight of the 10 diabetic patients had an
elevated HbA1c of more than 5.7%. This study was
approved by the Local Ethical Committee of Naresuan
University (permission number 4602040009)
and written informed consents were obtained from
all diabetic patients involved.
blood mononuclear cells (PBMC) stimulated with
virulent B. pseudomallei and avirulent B. thailandensis
was assessed in diabetic patients and compared to
that of healthy individuals. In addition, IL-23 gene
expression was investigated for its involvement in
IL-17 expression.