Finally 500 μL of ไอโซโพรพิลแอลกอฮอล์ and 50 μL
3 M NaAc were added to the upper phase and incubated at −20 °C to
precipitate the DNA. After centrifugation at 12,000 g for 10 min and
washing the precipitate twice in 70% ethanol, the resulting DNA was dissolved in TE buffer solution and stored at 20 °C prior to evaluation