For enhanced education at the level of University courses such as those in biology or biotechnology, one of the key elements is the need of time and expertise to allow the student to familiarize laboratory techniques in par with regular theory. The Sakshat Amrita virtual biotechnology lab project focusing on virtualizing wet-lab techniques and integrating the learning experience has added a new dimension to the regular teaching courses at the University. Establishing virtual labs requires both domain knowledge and virtualizing skills via programming, animation and device-based feedback. Challenges in the biotechnology sector in setting up a laboratory that integrates both the feel and phenomenon includes the medley of multiple techniques. This paper reports one such cost-effective process used in virtualizing a real biotechnology lab at the University-level. The major challenge in setting up an effective knowledge dissemination for laboratory courses was not only the scientific approach of biotechnology, but included the virtualization aspects such as usage/design scalability, deliverability efficiency, network connectivity issues, security and speed of adaptability to incorporate and update changes into existing experiments. This paper also discusses an issue-specific case-study of a functional virtual lab in biotechnology and its many issues and challenges.