Twelve pairs of adult A. royi were sorted from the acclimation beakers
and cultivated on 6-well culture plates at designed salinities
(0, 10, 20, and 30). The cultures were placed in the thermostatic incubator
programmed to the same conditions as those in the population
experiments. Nauplii and clutch production were recorded from daily
stereomicroscopic observations of the culture wells for 14 days. After
each observation, the pairs of copepods were transferred to new culture
wells containing fresh culture water and algal diets.