Absence of clear zones found in three (CT 1, CT 2 and CT 3) actinomycetes isolates during final examination of chitin agar plates (table 4), shows that no antibiotics were produced in such colonies. Although, the other three isolates (CT 4, CT 5 and CT 6) were able to inhibit the growth of indicator bacteria, however the inhibition was not observed in all tested indicator bacteria. This therefore means that there was a low secretion of antibiotics by such actinomycetes isolates. A probable explanation for these results could be that the culture method used in this study, did not provide ideal conditions that should have enabled the growth of large numbers of actinomycetes and secretion of high amounts of antibiotics. Furthermore, experiments with this technique (culture-dependent methods) are quite limited in detecting the broad population of uncultured microorganisms in the soil ecosystem [6].