Sub-lethal concentration of Cd affected the expression of HSP
genes in the liver of experimental fish (Fig. 2). Among the HSPs,
HSP47, HSP60, HSP78, and HSP90 was significantly up regulated in
the Cd-exposed group (Fig. 2); however, there were variations in
the level of expression among these HSPs. Transcription level of
HSP70 was insignificantly higher in the Cd-exposed as compared to
the control. Furthermore, we observed that gene expression levels
corresponded to protein levels of each respective gene. Moreover,
to verify the results of gene expression, immunoblotting validated
the observed transcriptional abundance of HSP60 and HSP90
Sub-lethal concentration of Cd affected the expression of HSPgenes in the liver of experimental fish (Fig. 2). Among the HSPs,HSP47, HSP60, HSP78, and HSP90 was significantly up regulated inthe Cd-exposed group (Fig. 2); however, there were variations inthe level of expression among these HSPs. Transcription level ofHSP70 was insignificantly higher in the Cd-exposed as compared tothe control. Furthermore, we observed that gene expression levelscorresponded to protein levels of each respective gene. Moreover,to verify the results of gene expression, immunoblotting validatedthe observed transcriptional abundance of HSP60 and HSP90
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