The probiotic culture L. acidophilus La-14 (Danisco) was activated
in MRS broth (Himedia) and incubated for 15 h at 37C.
Then, it was centrifuged at 4670 g for 15 min and washed with
NaCl solution (0.85%). The cells were suspended in saline to obtain a
solution containing about 10 log CFU g1. The concentration of
microorganism was adjusted by bacterial growth curve