The total dietary fibre (TDF) content of pastaswas determined using
theMegazyme International total DF assay and following the procedures
of Sun-Waterhouse et al. (2010). The protein content of the pastas was
determined using the Bradford colorimetric assay (Bradford, 1976).
Aqueous BSA solutions at 5 different concentrations (ranging from 0 to
1000 μg/mL) were used as standards to establish a calibration curve.