The total antioxidant activity was obtained by a modified DPPH
method (Brand-Wiliams, Cuvelier, & Berset, 1995) consisting in the
capture of the free radical 2,2-diphenyl-1-picryl-hydrazyl through
antioxidants. A solution of methanol containing 0.06 mM DPPH
was prepared. After adjusting with methanol an amount of
100 lL of extract was added to 3.9 ml of this solution. Following
that, to determine the reading time, a preliminary study was made
observing the absorbance reduction at 515 nm in 1 min intervals
for the first 15 min and then in 5 min intervals until stabilization,
for a total time of 125 min for the readings. The antioxidant activity
was expressed as the concentration of antioxidants required to
reduce the original amount of free radicals in 50% (EC50). The
results were expressed in g fruit/g DPPH.
The total antioxidant activity was obtained by a modified DPPHmethod (Brand-Wiliams, Cuvelier, & Berset, 1995) consisting in thecapture of the free radical 2,2-diphenyl-1-picryl-hydrazyl throughantioxidants. A solution of methanol containing 0.06 mM DPPHwas prepared. After adjusting with methanol an amount of100 lL of extract was added to 3.9 ml of this solution. Followingthat, to determine the reading time, a preliminary study was madeobserving the absorbance reduction at 515 nm in 1 min intervalsfor the first 15 min and then in 5 min intervals until stabilization,for a total time of 125 min for the readings. The antioxidant activitywas expressed as the concentration of antioxidants required toreduce the original amount of free radicals in 50% (EC50). Theresults were expressed in g fruit/g DPPH.
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