Selecting yeasts with inhibition effect on OTA-producing fungi
was performed in an agar media by the method of Visser, Holzapfel,
Bezuidenhout, and Kotze (1986) with some modifications. First,
10 mL of the prepared fungal spore suspension (106 spores/mL) was
mixed with 90 mL of PDA (45 C) and then poured onto five Petri
dishes. A yeast plug (0.7 cm in diameter) from the 2 d culture in YPD
was placed in the prepared fungal spore-containing PDA plates. A
plug of YPD medium without yeast was placed in the fungal sporecontaining
PDA medium and set as the control. All prepared PDA
plates were incubated at 25 C for 3 d.