The viscosity-average molecular weight, Mv, was determined
with an Ubbelohde viscosimeter (Technico, ASTM D. 445) at room
temperature. The solvent used for chitin was dimethylacetamide/
LiCl and for chitosan, 0.2 M acetic acid/0.3 M sodium acetate. The
viscosity-average molecular weight (Mv) was obtained from
viscosity equation using MarkeHouwink parameters according to
the equation: