Although producing biodiesel from microalgae seems promising, there is still a lack of technology for the
quick and cost-effective conversion of biodiesel from wet microalgae. This study was aimed to develop a
novel microalgal biodiesel producing method, consisting of an open system of microwave disruption, partial
dewatering (via combination of methanol treatment and low-speed centrifugation), oil extraction,
and transesterification without the pre-removal of the co-solvent, using Chlamydomonas sp. JSC4 with
68.7 wt% water content as the feedstock. Direct transesterification with the disrupted wet microalgae
was also conducted. The biomass content of the wet microalgae increased to 56.6 and 60.5 wt%, respectively,
after microwave disruption and partial dewatering. About 96.2% oil recovery was achieved under
the conditions of: extraction temperature, 45 C; hexane/methanol ratio, 3:1; extraction time, 80 min.
Transesterification of the extracted oil reached 97.2% conversion within 15 min at 45 C and 6:1 solvent/
methanol ratio with simultaneous Chlorophyll removal during the process. Nearly 100% biodiesel
conversion was also obtained while conducting direct transesterification of the disrupted oil-bearing
microalgal biomass.
Although producing biodiesel from microalgae seems promising, there is still a lack of technology for thequick and cost-effective conversion of biodiesel from wet microalgae. This study was aimed to develop anovel microalgal biodiesel producing method, consisting of an open system of microwave disruption, partialdewatering (via combination of methanol treatment and low-speed centrifugation), oil extraction,and transesterification without the pre-removal of the co-solvent, using Chlamydomonas sp. JSC4 with68.7 wt% water content as the feedstock. Direct transesterification with the disrupted wet microalgaewas also conducted. The biomass content of the wet microalgae increased to 56.6 and 60.5 wt%, respectively,after microwave disruption and partial dewatering. About 96.2% oil recovery was achieved underthe conditions of: extraction temperature, 45 C; hexane/methanol ratio, 3:1; extraction time, 80 min.Transesterification of the extracted oil reached 97.2% conversion within 15 min at 45 C and 6:1 solvent/methanol ratio with simultaneous Chlorophyll removal during the process. Nearly 100% biodieselconversion was also obtained while conducting direct transesterification of the disrupted oil-bearingmicroalgal biomass.
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