Oxidation of exposed sulfhydryl groups on hemoglobin causes formation of disulfide bonds and distortion of the tertiary structure of the hemoglobin molecule. The result is precipitation of hemoglobin, which may then coalesce to form intracellular inclusions called Heinz bodies. Small, non-pathologic “endogenous” Heinz bodies can be found in blood of many healthy cats, but their presence is abnormal in other species. Increased numbers of these “endogenous” Heinz bodies are found in cats with diabetes mellitus (associated with ketone production), lymphoma and hyperthyroidism. Although many of these cats are not anemic, their red blood cell lifespans are reduced and they have lower hematocrits than cats with the same disease but lower numbers of Heinz bodies.
Heinz bodies may be seen in Wright’s stained smears, especially if they are large and distort the red cell membrane. In contrast, small “endogenous” Heinz bodies, such as those that can be seen in cats, are harder to visualize. Heinz bodies can be easily observed with supravital stains, such as new methylene blue, as illustrated in the picture below.
Oxidation of exposed sulfhydryl groups on hemoglobin causes formation of disulfide bonds and distortion of the tertiary structure of the hemoglobin molecule. The result is precipitation of hemoglobin, which may then coalesce to form intracellular inclusions called Heinz bodies. Small, non-pathologic “endogenous” Heinz bodies can be found in blood of many healthy cats, but their presence is abnormal in other species. Increased numbers of these “endogenous” Heinz bodies are found in cats with diabetes mellitus (associated with ketone production), lymphoma and hyperthyroidism. Although many of these cats are not anemic, their red blood cell lifespans are reduced and they have lower hematocrits than cats with the same disease but lower numbers of Heinz bodies.Heinz bodies may be seen in Wright’s stained smears, especially if they are large and distort the red cell membrane. In contrast, small “endogenous” Heinz bodies, such as those that can be seen in cats, are harder to visualize. Heinz bodies can be easily observed with supravital stains, such as new methylene blue, as illustrated in the picture below.
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