The concept and operation of the TIS used in the exper- iments were based on the RITA® vessel developed by CIRAD [16] and made up of two compartments of two compartments of 250 ml of capacity each one (Figure 1(a)). Four shoots with two pairs of fully expanded leaves were inoculated per vessel, containing 225 ml of liquid culture proliferation medium each. The system was pro- grammed to transfer the medium and to immerse the ex- plants for 1 minute every 6 h. Three concentrations of BA (6.0, 12.0, and 18.0 μM) and a cytokinin-free me- dium (CK-free medium) were assayed. In order to test the profitability of the TIS culture system in relation to conventional culture methods, a control treatment con- sisting of shoots cultured on a conventional liquid basal MS medium (SS) supplemented with BA (12.0 μM) was included. The number of normal shoots (NS), number of hyperhydric shoots (HS), shoot length (cm) and number of leaves per shoot were recorded after 4 weeks of cul- ture. Three culture vessels were used in each treatment and the experiment was repeated three times.