MATERIALS AND METHODS
This study was conducted at the Research Laboratories, Department of Biochemistry and
Molecular Biology, Jahangirnagar University, Savar, Dhaka.
Extraction and formulation of Paederia foetida leaves: Paederia foetida leaves were first
cut and grinded and then subjected to mild hot continuous percolation for 12 hours using
absolute ethanol as solvent in a soxhlet apparatus. Then the extract was concentrated under
reduced pressure at 50ºC using rotary evaporator. The concentrated extract was further dried
by placing within desiccators (approximate yield 10%, w/w). Dried ethanol-free extract of
Paederia foetida was dissolved in physiological saline to make a homogenous solution of 15
mg/ml. The study was carried out in the laboratory of Department of Biochemistry and
Molecular Biology, Jahangirnagar University.