The digests were thawed to room temperature and their pH adjusted to 6.0. They were then incubated in a boiling water bath for 5 min to quench any enzyme activity followed by centrifugation
The digests were thawed to room temperature and their pHadjusted to 6.0. They were then incubated in a boiling water bathfor 5 min to quench any enzyme activity followed by centrifugation