Preparation of the extract Five hundred grams of air dried powdered from the leaves of L. tomentosa or D. virginiana was prepared and extracted separately with 6 liters of methanol 70% at room temperature 3 times until exhaustion by maceration. The extracts were concentrated under reduced pressure to give 54 and 58 g of crude extract respectively.
Phytochemical analysis The plant extract was tested for the presence of bioactive compounds according to the following standard tests: Molisch's test for carbohydrates, Shinoda test for flavonoids, Forth test for saponins, Farnsworth test for coumarins, Salkowski's test for terpenes and sterols,