Measure the subzero properties of frozen dough by dynamic mechanical thermal analysis (DMTA) and showed that the Tg measured by DMTA moved to higher temperature during frozen storage when at the optimal water content of dough. A reduction in water content eliminated this phenomenon. Frozen storage increase the liquid phase in dough with optimal water 500 BU. The growth of ice crystal during frozen storage resulted in the concentration of polymers and a higher Tg as observed by DMTA . The increase in the liquid phase during storage was substanially lower when the water content of dough was decreased and thus ice crystal growth was minimized