tIn the last few years, an increasing interest has been developed to the potential use of plant waste asraw material to produce natural dye for dyeing textiles due to environmental aspects. The current studydeals with the solvent extraction of natural dyes from three native plant species such as Mangifera indica,Glochidion lanceolarium and Litsea sebifera, statistical analyses, and dyeing techniques on silk and cottonyarn using cationic and anionic surfactant. The dyed yarns displayed excellent antimicrobial activityagainst the bacterial strains for the development of antimicrobial textiles. The interaction of natural dyeswith a cationic surfactant, cetyltrimethyl ammonium bromide, and an anionic surfactant, sodium dodecylbenzene sulphonate, found to be significant. The colour strength of the dyed yarn using cationic surfactantwas found to be better and higher than the anionic surfactant. From the dye absorption, it was observedthat the dye intensity was enhanced with the increase in dye absorption by the yarns. The reports foreseedeveloping a sustainable technology for utilization of waste bio-resources for the economic growth ofthe rural weaver’s societies in India.