2.4. Extraction and methanolysis of fixed oil
The seeds were carefully obtained from the fruits and airdried.
30 g each of the air-dried seeds were pulverized and
extracted with hexane for about 5 h in a soxhlet extractor.
The oil extracts were concentrated under reduced pressure.
Oil (1 mL) was dissolved in 20 mL petroleum ether and 2 mL
methanolic KOH (2 M). The mixture was shaken for 2 min
and allowed to stand for about 30 min. The upper layer was
removed and washed with water[11]. The resulting Fatty acid
methyl esters (FAME) were subjected to GC-MS analysis.