special high pressure dosing pump for dosing DMDC
is necessary. Due to limited availability of such
equipments, the DMDC in CSP was homogenized
using an automatic stirrer with a blunt paddle at
speed 830 rpm for 5 minutes. However, such method
was not sufficient to ensure well distribution of
DMDC in the CSP. The DMDC was less effective
in the CSP dispersion and probably did not dissolve
quickly enough to act on the microorganisms (Lay,
2009). Another reason which might have reduced the
efficiency of the DMDC in this case is the availability
of fat in the CSP. According to the findings of Mah
Hassan (2008), the percentage of fat in CSP is around
0.41% and the protein content is around 7.67%.
The lethal mechanism of DMDC works by entering
microbial cell wall as it is a lipophilic compound
and inactivates various enzymes such as by reacting
with imidazol groups, thereby deactivating the
microorganism. If there are fat or oil droplets present,
DMDC will enter the oil droplet and gradually
hydrolyzes at the interface resulting in an efficacy
drop for microbial inactivation (Lay, 2009).