Transplantation procedure
24 hours after cerebral ischemia, animals received either C17.2 neural stem cells or phosphate buffered saline (PBS). Animals were anesthetized with 3% sodium pentobarbital and then approximately 5×106 NSCs in a volume of 200 µl PBS or equal volume of PBS alone were injected into the rat tail vein at 24 hours after stroke. Immunosuppressants were not used in any animals.