Owing to the high therapeutic potential, it is of high stipulate in pharmaceutical industries which escort to its overexploitation resulting in the relapsing of the population of C.asiatica to a precarious level that a ban on its collection from their natural habitat has been thus recognized as threatened medicinal herb is endermuc to Western Ghats of South india.Thus,it has become imperative to develop alternative approaches for its conservation,in order to protect it from the verge of extinction.In vitro culture technique offers a viavle tool for the mass propagation of plant and conservation of rara,threatened and endangered germplasm.Therefore,in the present study, an attempt had been made for in vitro plantlet regeneration of C.asiatica through callus mediated organogenesis