Mouth
Do not put fingers or other objects such as pens, in the mouth
Do not use mouth pipetting
Eating, drinking, smoking is not permitted in any working area of the
laboratory No food or beverage be stored in any laboratory refrigerator, freezer,
hot air oven or incubator
Avoiding needle stick injuries
Never recap or bend needles
Dispose all sharps in puncture proof containers
In case of a needle stick injury, squeeze out the blood, wash the hand
with the anti-septic and make out an incident report Post exposure prophylaxis as per guidelines under State AIDS Control
Programme DISPOSAL OF BLOOD AND LABORATORY MATERIALMethod of Disposal of Blood BagsShould comply with requirements of
Biomedical Wastes Rules of Ministry of Environment and Forests and local
pollution control board Needles should be burnt using electric needle destroyers or soaked in
hypochlorite solution or discarded in a puncture proof container of a
non-chlorinated plastic. These should then be sent for deep burial or
incineration
Q-3.3 Disinfection of glassware: All reusable glassware should be disinfected
by treating with hypochlorite and detergent before cleaning. Subsequently
glassware can go to hot air oven at 1600C for 1 hour.
Q-3.4 Spills on the table tops/sinksThe spill should be covered with filter papers
or plain cloth and soak with 1% hypochlorite solution for at least 30
minutes and later swabbed Hypochlorite – detergent solution0.5-1.0 per cent solution of hypochlorite
is the best general purpose disinfectant if contact is maintained for at
least 30 minutes. (Except for metallic equipment which could be autoclaved
or put in cidex) Disposal by SterilisationAutoclaving for 30 minutes at 1210C and 15 p.s.i.
(68.5 cm Hg) is the method of choice. Validation with use of biological
indicator (B. stereothermophilus) should be done at least once a month
Mouth Do not put fingers or other objects such as pens, in the mouth Do not use mouth pipetting Eating, drinking, smoking is not permitted in any working area of thelaboratory No food or beverage be stored in any laboratory refrigerator, freezer,hot air oven or incubator Avoiding needle stick injuries Never recap or bend needles Dispose all sharps in puncture proof containers In case of a needle stick injury, squeeze out the blood, wash the handwith the anti-septic and make out an incident report Post exposure prophylaxis as per guidelines under State AIDS ControlProgramme DISPOSAL OF BLOOD AND LABORATORY MATERIALMethod of Disposal of Blood BagsShould comply with requirements ofBiomedical Wastes Rules of Ministry of Environment and Forests and localpollution control board Needles should be burnt using electric needle destroyers or soaked inhypochlorite solution or discarded in a puncture proof container of anon-chlorinated plastic. These should then be sent for deep burial orincinerationQ-3.3 Disinfection of glassware: All reusable glassware should be disinfectedby treating with hypochlorite and detergent before cleaning. Subsequentlyglassware can go to hot air oven at 1600C for 1 hour.Q-3.4 Spills on the table tops/sinksThe spill should be covered with filter papersor plain cloth and soak with 1% hypochlorite solution for at least 30minutes and later swabbed Hypochlorite – detergent solution0.5-1.0 per cent solution of hypochloriteis the best general purpose disinfectant if contact is maintained for atleast 30 minutes. (Except for metallic equipment which could be autoclavedor put in cidex) Disposal by SterilisationAutoclaving for 30 minutes at 1210C and 15 p.s.i.(68.5 cm Hg) is the method of choice. Validation with use of biologicalindicator (B. stereothermophilus) should be done at least once a month
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