The measure quantity of 25 L of each concentration was
pipetted out with a sterilized pipett and filed in the wells
aseptically. In the control plate only Tween- 80 was added
into the well. The oil was allowed to defuse in the well for a
period of one hour and plates were incubated at
for 24-48 hours. The zone of inhibition (mm) was measured
with graduated scale after the period of incubation.