To prepare TSB, the following ingredients are dissolved under gentle heat and then autoclaved for 15 minutes at 121 °C (250 °F).
17 grams (0.60 oz) of Tryptone
3 grams (0.11 oz) of Phytone
5 grams (0.18 oz) of NaCl
2.5 grams (0.088 oz) of dipotassium phosphate (K2HPO4)
2.5 grams (0.088 oz) of glucose
1 liter (35 imp fl oz; 34 U.S. fl oz) of distilled water
Phytone, which is an enzymatic digest of soybean meal.