The conclusions of this study are
Airlift bioreactor had superior performance on the growth and lipid production of R.glutinis as compared to the agitation tank
Fed-batch operation,at crude glycerol concentration of feeding,could enhance cell growth and lipid production
Expernential feeding criteria could avoid the accumulation of high crude glycerol concentration,and led to the enhancement of cell growth rate.However,the simple pulse feeding criteria achieved the highest total lipid and β-carotene productivity.
From the perspective of collecting the maximum total lipids for biodiesel production, pulse feeding was suggested as a suitable criteria for the cultivation of R.glutinis with crude glycerol