The Sub-middle region of the São Francisco Valley (SFV), located in the Northeast of Brazil at latitude 8 to 9°S and longitude 40 W, with a tropical semi-arid climate, has received considerable interest from the wine production sector. This region is the second biggest producer of refined grapes and wines in Brazil, and represents more than 95% of the national grape exportation (Mello, 2013). Recently, there has been notable investment in the large-scale commercial production of grape juice in this region. An important difference between the tropical viticulture practised in the SFV and that of other traditional regions of the world is that in the SFV each vine can produce two harvests per year. Also, since this is a region with hot weather, high luminosity and abundant water for irrigation, wineries operate according to a particular scheme, evaluating the best period in which to harvest the grapes and to prune the vines. Also, the “step” system can be applied, where the harvesting is distributed within a certain period (e.g., one month, several months or the whole year) (Camargo, Tonietto, & Hoffmann, 2011). Thus, it is possible to prepare grape juices throughout the year.