Duplicate samples of influent and effluent liquid were collected
from the ANAMMOX reactor at the same time each day,
during the 80 day test period. NH4
+-N was analysed using the
Nessler’s Reagent Spectrophotometric technique; NO2
−-N was
analysed using a Diazo coupled spectrophotometric technique; and
NO3
−-N was measured using ultraviolet (UV) spectrophotometry
methods[10]. Temperature, pH and DO were measured using a
WTW pH/DO meter (WTW Multi 340i, Germany). The nitrogen
removal rate (kg N m−3 day−1) was calculated as the daily nitrogen
load removed [daily flow rate × (Nin−Nout)] per unit volume of
the reactor