Bauer et al. reported that derivatization with diazomethane is not free from artefacts, and characterized these by GC–MS [266]. A significant difference in results between conventional methods of measuring FFAs in plasma, and methods utilizing diazomethane, was reported by Chu et al. [267]. The results of Chu et al. are not surprising when we take into account that water and inorganic salts react with diazomethane, causing the solution to become basic. This can lead to the methanolysis of lipids. The subject is clearly reviewed by Schmid and Schmid [268].