the separation of phases was performed by centrifugation using a centrifuge (Bioblock scientific 2–5, SIGMA) at 3500 rpm then filtered through a 0.2μm cellulose nitrate filter (Sartorius).
the separation of phases was performed by centrifugation using acentrifuge (Bioblock scientific 2–5, SIGMA) at 3500 rpm thenfiltered through a 0.2μm cellulose nitrate filter (Sartorius).