To avoid errors in the judgment and reduce investment
Zoheir and Allam [9] added EB or CuSO4 after
the LAMP reactions in bovine sexing techniques, The
authors did these additions in the present work. The
addition of EB 1mM (1:5 v/v) after the LAMP
reaction to the specimens tubes, leads to color changes;
the salmon pink a positive reaction (male) and wine
coloration is a negative reaction and female as reported
[9]. The intensity of the color depends mainly on the
concentration of DNA after the LAMP reaction.