Changes in gelatinase activity during follicle development
Gelatinase activity in the ovaries of normal and miniature pigs was evaluated using in situ zymography (Fig. 2). Overall, gelatinase activity was higher in the ovaries of normal pigs than in those of miniature pigs. During follicular development, similar gelatinase activity was observed in both breeds. In the Graafian follicles, gelatinase activity was high in normal pigs, especially in theca interna cells (Fig. 2A-1); in contrast, in miniature pigs, gelatinase activity was insignificant, with minimal activity in theca interna cells (Fig. 2B-1). Although gelatinase activity decreased after ovulation, it was still higher in normal pigs than in miniature pigs. Compared to high gelatinase activity in the Graafian follicles and theca interna, the activity observed in the theca externa was relatively low. After ovulation, low gelatinase activity was detected in the granulosa lutein cells of the luteinising follicles.
Changes in gelatinase activity during follicle developmentGelatinase activity in the ovaries of normal and miniature pigs was evaluated using in situ zymography (Fig. 2). Overall, gelatinase activity was higher in the ovaries of normal pigs than in those of miniature pigs. During follicular development, similar gelatinase activity was observed in both breeds. In the Graafian follicles, gelatinase activity was high in normal pigs, especially in theca interna cells (Fig. 2A-1); in contrast, in miniature pigs, gelatinase activity was insignificant, with minimal activity in theca interna cells (Fig. 2B-1). Although gelatinase activity decreased after ovulation, it was still higher in normal pigs than in miniature pigs. Compared to high gelatinase activity in the Graafian follicles and theca interna, the activity observed in the theca externa was relatively low. After ovulation, low gelatinase activity was detected in the granulosa lutein cells of the luteinising follicles.
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