Firstly, by the use of hexane/benzene (70:30 v/v) chromarods
were dried and partially scanned to determine apolar lipids. In order
to determine neutral lipids, the development in benzene/chloroform/
formic acid (70:25:1 v/v) was performed. Finally, the determination of
polar lipids was achieved by the development in chloroform/acetone/
methanol/acetic acid/ water (30:40:10:10:5 v/v).