DNA in shoots and roots from 4 and 8 DPI
on. The greatest differences among genotypes
were found at 12 to 16 DPI.
A high correlation was found with MTAS-
ELISA and squash blot with the
symptomatology in shoot apex samples in
both genotypes (Fig. 2). However, M-TASELISA
failed to reliably detect TYLCV in
early and late stages of infection, resulting
in a high number of inconclusive results.
M-TAS-ELISA gave unexpectedly high
values in roots samples, which do not allow
accurate infection monitoring and do
not differentiate between the resistance
levels of FC and BC2-chil as accurately as
squash blot.