Mueller Hinton Agar (MHA)
The commercially available MHA
purchased from HiMedia Pvt. Ltd, Mumbai, India was
used in this study.
Phomopsis sp. GJJM07 in different media
To prepare inocula for fermentation studies,
mycelial discs (9 mm) of 7 days old culture of
Phomopsis sp. GJJM07 were removed and transferred
aseptically into each 250 ml Erlenmeyer flask
containing 100 ml of PDB, PDYEB, M-1DB, MEB
and CDB medium . The seed flasks were incubated at
23 ± 2°C for 28 days under static condition. After
incubation, the growth of fungi was measured by
means of mycelial dry weight.
Extraction and antimicrobial activity