Algal mass productions are widespread events throughout the world. Most research deals with the environmental impact, ecology and toxicity of these phenomena, but the algae are also promising sources of bioactive natural products, and also are potential food additives. In the current study, we aimed to characterize the carotenoid composition of three non-toxic algal isolates with distinct taxonomical position, namely Dunaliella salina, Euglena sanguinea and a Nostoc strain.