Since the glucose concentration was 10.1 mmol/L, acetic acid production according to Eq. (3) should reach 12 mmol/L or 10.1 mmol/L of butyrate considering a yield of 59% (2.4 mol H2/m3) which would be 100% yield according to Eq. (4). The suppression of methanogens was followed by concomitant production of acetic- and butyric-acid followed by propionic acid (after a lag phase) soon after the initial substrate feeding