After 1 h, mice were sacrificed
and pulmonary hemorrhage observed. The MPHD of venom
preincubated with batimastat was then determined. Samples
of pulmonary tissue were obtained by dissection, added to
Karnovsky’s fixative, and routinely
processed for embedding in Spur resin. Thick (1 mm)
sections were prepared and stained with toluidine blue for
histological analysis.