Chemical and biopreservatives influenced the physico-chemical, microbial and sensory properties of Cheddar cheese during ripening storage. Nisin demonstrated positive influence on most of sensory characteristics of Cheddar cheese compared to other biopreservatives such as lactic acid bacteria and lysozyme and chemical preservative NaNO3. Incorporation of nisin did not nega- tively influence the microbial and major physico-chemical properties of Cheddar cheese. Thus, nisin could be a suitable option in preserving Cheddar cheese without affecting its quality characteristics.