The resulting culture was filtered stated above [10].
through cheesecloth to remove mycelial fragments,
washed by centrifugation (10,000 rpm for 15 min) and Preparation of Antagonist Suspension of S. griseus for
resuspended in sterile distilled water. microconidial
suspension of F. oxysporum f. sp. lycopersici, final
density of 10 microconidia/5 ml was inoculated in to sand
maize medium and it was incubated at room temperature
for 14 days. For green house experiment, the substrate at
5 percent (w/w) was mixed with sterilized soil and infested
into the pot ten days prior to transplanting the 60-day-old
seedling transplantation. The population of F. oxysporum
f. sp. lycopersici in the substrate was estimated as
number of colony forming units (CFU) per gram of
substrate, as previously described [6]. Approximately 2 x
108 cfu/g of innoculum of F. oxysporum f. sp. lycopersici
was used for pot experiments.