Fatty acid methyl esters (FAME) were prepared by a standard
method as described by AOCS (1998) using an internal standard
(C11:0, 5.65 g/100 g). FAMEs were separated on a GC (HP 5890)
system using Agilent-Technologies DB-Wax capillary column (30 m
long, with internal diameter 0.25 mm, film thickness 0.25 μm).