In comparison to the chemical hydrolysis, enzymatic hydrolysis is preferable due to several advantages, such as mild reaction conditions, low undesirable products, and high product quality and yield. Alcalase has been found to be a highly efficient enzyme in fish protein due to its ability to attain a high degree of hydrolysis in a relatively short period under mild conditions (Aspmo et al., 2005) and can produce fish protein hydrolysate with good nutritional and functional properties