m
m
starch content and sugar content.
Respiration rate was determined in three replicates each
containing three fruit at each sampling times. Three fruit
were placed in a 2-L desiccator and held under vacuum
for 10 min to remove the internal air (Liu et al. 2004). The
fruits were then allowed to rest in air for 35 min before being
sealed inside a 2-L jar for 2.5 h at 20°C. 1 mL sample of
headspace gas was withdrawn by an airtight syringe and
injected into a gas chromatography (GC) (G-3900; Hitachi
Corp, Japan). The GC was equipped with a thermal conductivity
detector(TCD)and a Porepak Q column. the flow
rate
of
the
carrier
helium gas was 40 mL
min
. The oven
was set at 60°C and the injector was kept at 125°C.
The analysis of ethylene production rate was carried
out in gas chromatography (Model GC-17A; Shimadzu Co.
Kyoto,japan)fitted whit a flame ionization detected(FID)
and an activated alumina column, with an injector temperature
of 120°C, column temperature
of
60°C, and detector
temperature
of
60°C. The
rate of ethylene production
was