Nodal segments with one bud and 10 mm long were used, originated from three successive subcultures in MS medium
(MURASHIGE; SKOOG, 1962) supplemented with 100 mg L-1 of myo-inositol, 30 g L-1 of sucrose and 7 g L-1 of agar, pH 5.8, cultivated in growth room under white fluorescent lamps.