A new dye-decolorizing white-rot fungus was isolated and identified as Trametes trogii based on its ITS-
5.8S rRNA gene sequence analysis and morphological characteristics. Laccase was the only lignolytic
enzyme produced by this strain during solid substrate fermentation (SSF) in soybean cake, a solid agroindustrial
residue used for the first time in enzyme production. The extracellular crude enzyme from T.
trogii in solid substrate fermentation showed good activity in synthetic dye color removal, decolorizing
85.2% Remazol Brilliant Blue R (50 mg l−1), 69.6% Reactive Blue 4 (35 mg l−1), and 45.6% Acid Blue 129
(83.3 mg l−1) without the addition of redox mediators, 90.2% Acid Red 1 (10 mg l−1), and 65.4% Reactive
Black 5 (18.3 mg l−1) with the addition of 1 mM 1-hydroxybenzotriazole in 30 min. Native polyacrylamide
gel electrophoresis (Native-PAGE) of the crude enzyme and effects of laccase inhibitors on decolorization
corroborated the laccase as the major enzyme involved in the decolorization of dyes. The comparison
of color removal by the crude culture filtrates and by the whole fungal culture on the solid substrate
revealed the former was more advantageous.